Campbell-Brown, a two-time Olympic 200-metre champion, crashed out after finishing third in Heat 9 of the event on Monday.
The 34-year-old Campbell-Brown was no match for Edidiong Ofonime Odiong of Bahrain and Trinidadian Semoy Hackett.
Both Odiong and Hackett came off the curve in full control, leaving Campbell-Brown to struggle to the line in 22.97, a time not good enough to qualify her to the semi-finals as one of the sixth fastest losers.
Odiong won the heat in a personal best 22.74, while Hackett clocked 22.78 for the other automatic qualifying spot from the heat.
Campbell-Brown, affectionately called VCB, is a seven-time Olympic medallist and the second woman in history to win two consecutive Olympic 200-metre events.
She is competing in her fifth Olympics after making her debut at the Games 16 years ago in Sydney.
The other two Jamaicans – Simone Facey and Elaine Thompson – are safely through to the semi-finals.
Thompson, Jamaica’s newest sprint champion, and Facey booked their spots in in the semi-final after placing second in their heats.
Thompson, who won the 100 Olympic title on Saturday, was second in her heat in 22.63 behind Jose-Marie Ta Lou of Ivory Coast (22.31, a personal best).
And Facey clocked 22.78 for her second place behind Trinidadian Michelle-Lee Ahey, who won in 22.50
Ta Lou’s time of 22.31 seconds is the fastest going into the semi-finals, followed by Americans Deajah Stevens in 22.45 and Tori Bowie, the silver medalist in the 100, in 22.47.
World champion Dafne Schippers won the first heat and advanced in 22.51.
The semifinals are Tuesday, and the final is on Wednesday night.
FEATURED PHOTO CAPTION: Jamaica’s Veronica Campbell-Brown (centre), British Virgin Island’s Ashley Kelly (right), and Kazakhstan’s Olga Safronova compete in a women’s 200-metre heat during the athletics competitions of the 2016 Summer Olympics at the Olympic stadium in Rio de Janeiro, Brazil, Monday, Aug. 15, 2016.
Source: Loop Jamaica